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human primary saecs  (ATCC)


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    ATCC human primary saecs
    Human Primary Saecs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+small+airway+epithelial+cells+saec/Primary+Small+Airway+Epithelial+Cells%3B+Normal%2C+Human/pmc12460833-46-4-20
    Average 97 stars, based on 1439 article reviews
    human primary saecs - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Blocking IL-23 Signaling Mitigates Cigarette Smoke-Induced Murine Emphysema.
    Article Snippet: Inflammatory cell infiltration is a characteristic feature of COPD and correlates directly with the severity of the disease.. Interleukin23 (IL23) is a proinflammatory cytokine that regulates Th17 inflammation, which mediates many pathophysiological events in COPD.. The primary goal of this study was to determine the role of IL23 as a mediator of key pathologic processes in cigarette smokeinduced COPD.

    In Vitro:

    Article Title: Blocking IL-23 Signaling Mitigates Cigarette Smoke-Induced Murine Emphysema.
    Article Snippet: Inflammatory cell infiltration is a characteristic feature of COPD and correlates directly with the severity of the disease.. Interleukin23 (IL23) is a proinflammatory cytokine that regulates Th17 inflammation, which mediates many pathophysiological events in COPD.. The primary goal of this study was to determine the role of IL23 as a mediator of key pathologic processes in cigarette smokeinduced COPD.

    other:

    Article Title: Triptolide inhibits Wnt signaling in NSCLC through upregulation of multiple Wnt inhibitory factors via epigenetic modifications to Histone H3
    Article Snippet: Human small airway epithelial cells (SAEC), A549, H460, H358, and H1299 cell lines were obtained from ATCC.



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    (A) Schematic of the PhysioMimix® lung MPS co-culture system. <t>Epithelial</t> cells were seeded on the apical side of the Transwell® insert and human pulmonary microvascular endothelial cells (HPMVECs) on the basolateral side, under air–liquid interface (ALI) and dynamic flow conditions. (B) Representative H&E and Alcian blue-stained histological section of bronchial MPS co-culture after 14 days. Mucus is stained blue; endothelial cells are indicated by white arrows. Scale bar, 50 µm. (C) TEER measurements comparing epithelial monoculture, endothelial monoculture, and epithelial–endothelial co-culture over 14 days under ALI conditions. (D) TEER comparison of bronchial and alveolar co-cultures grown under static or dynamic flow MPS conditions over the 14-day ALI differentiation period. (E) Gene expression of Club cell (SCGB1A1) and Goblet cell (MUC5AC) markers in NHBE cells before culture (NHBE pellet) and after 14 days of differentiation in MPS co- culture. (F) Gene expression of alveolar markers - AT1 (AQP5) and AT2 (SFTPB) - in <t>SAEC</t> cells before culture (SAEC pellet) and after 14 days of MPS co-culture. (G) Immunofluorescence staining of bronchial MPS co-culture tissue for acetylated α-tubulin (yellow), mucus (MUC5AC, green), actin (phalloidin, red), and nuclei (Hoechst 33342, blue). Top row shows epithelial layer; bottom row shows endothelial layer. Scale bar, 100 µm. (H) Immunofluorescence staining of alveolar MPS co-culture tissue after 14 days of differentiation, showing surfactant (SFTPB, green), actin (phalloidin, magenta), and nuclei (Hoechst 33342, blue). Endothelial cells are marked with white arrows. Scale bar, 20 µm.
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    (A) Schematic of the PhysioMimix® lung MPS co-culture system. <t>Epithelial</t> cells were seeded on the apical side of the Transwell® insert and human pulmonary microvascular endothelial cells (HPMVECs) on the basolateral side, under air–liquid interface (ALI) and dynamic flow conditions. (B) Representative H&E and Alcian blue-stained histological section of bronchial MPS co-culture after 14 days. Mucus is stained blue; endothelial cells are indicated by white arrows. Scale bar, 50 µm. (C) TEER measurements comparing epithelial monoculture, endothelial monoculture, and epithelial–endothelial co-culture over 14 days under ALI conditions. (D) TEER comparison of bronchial and alveolar co-cultures grown under static or dynamic flow MPS conditions over the 14-day ALI differentiation period. (E) Gene expression of Club cell (SCGB1A1) and Goblet cell (MUC5AC) markers in NHBE cells before culture (NHBE pellet) and after 14 days of differentiation in MPS co- culture. (F) Gene expression of alveolar markers - AT1 (AQP5) and AT2 (SFTPB) - in <t>SAEC</t> cells before culture (SAEC pellet) and after 14 days of MPS co-culture. (G) Immunofluorescence staining of bronchial MPS co-culture tissue for acetylated α-tubulin (yellow), mucus (MUC5AC, green), actin (phalloidin, red), and nuclei (Hoechst 33342, blue). Top row shows epithelial layer; bottom row shows endothelial layer. Scale bar, 100 µm. (H) Immunofluorescence staining of alveolar MPS co-culture tissue after 14 days of differentiation, showing surfactant (SFTPB, green), actin (phalloidin, magenta), and nuclei (Hoechst 33342, blue). Endothelial cells are marked with white arrows. Scale bar, 20 µm.
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    Schematic of the making of the small airway <t>epithelial</t> cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.
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    primary normal human small airway epithelial cells (saec)  (Lonza)
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    Schematic of the making of the small airway <t>epithelial</t> cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.
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    Schematic of the making of the small airway <t>epithelial</t> cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.
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    human small airway epithelial cells saec  (ATCC)
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    ATCC human small airway epithelial cells saec
    Schematic of the making of the small airway <t>epithelial</t> cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.
    Human Small Airway Epithelial Cells Saec, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+small+airway+epithelial+cells+saec/Primary+Small+Airway+Epithelial+Cells%3B+Asthma%2C+Human/pm39221838-82-0-8
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    Image Search Results


    (A) Schematic of the PhysioMimix® lung MPS co-culture system. Epithelial cells were seeded on the apical side of the Transwell® insert and human pulmonary microvascular endothelial cells (HPMVECs) on the basolateral side, under air–liquid interface (ALI) and dynamic flow conditions. (B) Representative H&E and Alcian blue-stained histological section of bronchial MPS co-culture after 14 days. Mucus is stained blue; endothelial cells are indicated by white arrows. Scale bar, 50 µm. (C) TEER measurements comparing epithelial monoculture, endothelial monoculture, and epithelial–endothelial co-culture over 14 days under ALI conditions. (D) TEER comparison of bronchial and alveolar co-cultures grown under static or dynamic flow MPS conditions over the 14-day ALI differentiation period. (E) Gene expression of Club cell (SCGB1A1) and Goblet cell (MUC5AC) markers in NHBE cells before culture (NHBE pellet) and after 14 days of differentiation in MPS co- culture. (F) Gene expression of alveolar markers - AT1 (AQP5) and AT2 (SFTPB) - in SAEC cells before culture (SAEC pellet) and after 14 days of MPS co-culture. (G) Immunofluorescence staining of bronchial MPS co-culture tissue for acetylated α-tubulin (yellow), mucus (MUC5AC, green), actin (phalloidin, red), and nuclei (Hoechst 33342, blue). Top row shows epithelial layer; bottom row shows endothelial layer. Scale bar, 100 µm. (H) Immunofluorescence staining of alveolar MPS co-culture tissue after 14 days of differentiation, showing surfactant (SFTPB, green), actin (phalloidin, magenta), and nuclei (Hoechst 33342, blue). Endothelial cells are marked with white arrows. Scale bar, 20 µm.

    Journal: bioRxiv

    Article Title: Dynamic Culture Improves the Predictive Power of Bronchial and Alveolar Airway Models of SARS-CoV-2 Infection

    doi: 10.1101/2025.07.21.665885

    Figure Lengend Snippet: (A) Schematic of the PhysioMimix® lung MPS co-culture system. Epithelial cells were seeded on the apical side of the Transwell® insert and human pulmonary microvascular endothelial cells (HPMVECs) on the basolateral side, under air–liquid interface (ALI) and dynamic flow conditions. (B) Representative H&E and Alcian blue-stained histological section of bronchial MPS co-culture after 14 days. Mucus is stained blue; endothelial cells are indicated by white arrows. Scale bar, 50 µm. (C) TEER measurements comparing epithelial monoculture, endothelial monoculture, and epithelial–endothelial co-culture over 14 days under ALI conditions. (D) TEER comparison of bronchial and alveolar co-cultures grown under static or dynamic flow MPS conditions over the 14-day ALI differentiation period. (E) Gene expression of Club cell (SCGB1A1) and Goblet cell (MUC5AC) markers in NHBE cells before culture (NHBE pellet) and after 14 days of differentiation in MPS co- culture. (F) Gene expression of alveolar markers - AT1 (AQP5) and AT2 (SFTPB) - in SAEC cells before culture (SAEC pellet) and after 14 days of MPS co-culture. (G) Immunofluorescence staining of bronchial MPS co-culture tissue for acetylated α-tubulin (yellow), mucus (MUC5AC, green), actin (phalloidin, red), and nuclei (Hoechst 33342, blue). Top row shows epithelial layer; bottom row shows endothelial layer. Scale bar, 100 µm. (H) Immunofluorescence staining of alveolar MPS co-culture tissue after 14 days of differentiation, showing surfactant (SFTPB, green), actin (phalloidin, magenta), and nuclei (Hoechst 33342, blue). Endothelial cells are marked with white arrows. Scale bar, 20 µm.

    Article Snippet: Airway models were comprised of human pulmonary microvascular endothelial cells (HPMEC) (PromoCell, C-12281, lot.489Z024.1) and small airway epithelial cells (SAEC) (Lonza, CC-2547, lot.21TL214918) or normal human bronchial epithelial cells (NHBE) (Lonza, CC-2540, lot.21TL035497) for the alveolar and bronchial airway model, respectively.

    Techniques: Co-Culture Assay, Staining, Comparison, Gene Expression, Immunofluorescence

    Schematic of the making of the small airway epithelial cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Schematic of the making of the small airway epithelial cell (SAEC) and human umbilical vein endothelial cell (HUVEC) model. SAEC cells are plated in a transwell insert with expansion media for 3–4 days. Once SAEC attach to the insert and become confluent, the media from the upper chamber is removed and the SAECs are exposed to air on the apical side and air–liquid interface (ALI)-S media on the basal side of the transwell. Media is changed every 2 days during the differentiation process. After approximately 4 weeks the SAECs at ALI differentiate into mature SAECs comprised of basal, club, goblet, and ciliated cells. The transwell insert is inverted and HUVECs are added and allowed to adhere overnight at 37 °C to the underside of the transwell insert using a sterile Eppendorf tube as a protective vessel for media and cells. Once the HUVECs attach, the transwell insert is flipped over and the HUVECs are exposed to 50/50 (volume %) ALI-S/HUVECs media while the SAECs are exposed to air. Created with BioRender.com.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Sterility

    Airway model with small airway epithelial cells (SAECs) on the apical side and human umbilical vein endothelial cells (HUVECs) on the basal side. ( A ) Confocal immunofluorescence microscopy images, visualized using LASX 3D Viewer, of the co-culture showing the differentiated SAEC cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green). Panel ( B ) depicts the apical SAEC layer and panel ( C ) the basal HUVECs (VE-cadherin-AF594 (yellow) layer of the model. Panel ( D ) (apical) depicts tight junctions (zonula occludens-1-AF647, green; E-cadherin-AF488, red; overlay in yellow). Panel ( E ) depicts the epithelial trans-epithelial electrical resistance (TEER). Panels ( F ) and ( G ) depict epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF-A) protein secretion for 2 independent experiments with 4 replicates per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Airway model with small airway epithelial cells (SAECs) on the apical side and human umbilical vein endothelial cells (HUVECs) on the basal side. ( A ) Confocal immunofluorescence microscopy images, visualized using LASX 3D Viewer, of the co-culture showing the differentiated SAEC cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green). Panel ( B ) depicts the apical SAEC layer and panel ( C ) the basal HUVECs (VE-cadherin-AF594 (yellow) layer of the model. Panel ( D ) (apical) depicts tight junctions (zonula occludens-1-AF647, green; E-cadherin-AF488, red; overlay in yellow). Panel ( E ) depicts the epithelial trans-epithelial electrical resistance (TEER). Panels ( F ) and ( G ) depict epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF-A) protein secretion for 2 independent experiments with 4 replicates per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Immunofluorescence, Microscopy, Co-Culture Assay

    Exposure of the small airway epithelial cells (SAECs) in the model to experimental rhinovirus 16 (RV16). Fold change in cytokine expression of SAECs following RV16 treatment compared to untreated control for ( A ) C-X-C motif chemokine ligand 10 (CXCL10), ( B ) soluble intercellular adhesion molecule-1 (sICAM-1), and ( C ) interleukin-6 (IL-6). ( D ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (control) and RV16 treated SAECs for 2 independent experiments with 4 replicates per experiment. ( E ) Confocal immunofluorescence microscopy images, visualized using LASX 3D Viewer, of tight junctions (zonula occludens-1-AF647, red; E-cadherin-AF488, green; overlay in yellow) for untreated (control; upper panel) and RV16 treated (lower panel) and ( F ) showing the differentiated SAECs cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green), for the untreated (upper panel), RV16 treated (middle panel), and RV16 plus lipopolysaccharide (LPS) treated SAECs (lower panel). ( G ) Volcano plot of the up-regulated (red circles) and down-regulated (blue circles) genes comparing RV16 treated versus untreated SAECs as measured by Q-PCR. Labeled genes show at least a twofold change in expression at a significance level (α) of less than 0.05. ( H ) Heatmap of the significant differentially expressed genes for the RV16 treated SAECs ( n = 5) and untreated controls ( n = 3) showing low expression (green) and high expression (red) for the individual samples. Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Exposure of the small airway epithelial cells (SAECs) in the model to experimental rhinovirus 16 (RV16). Fold change in cytokine expression of SAECs following RV16 treatment compared to untreated control for ( A ) C-X-C motif chemokine ligand 10 (CXCL10), ( B ) soluble intercellular adhesion molecule-1 (sICAM-1), and ( C ) interleukin-6 (IL-6). ( D ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (control) and RV16 treated SAECs for 2 independent experiments with 4 replicates per experiment. ( E ) Confocal immunofluorescence microscopy images, visualized using LASX 3D Viewer, of tight junctions (zonula occludens-1-AF647, red; E-cadherin-AF488, green; overlay in yellow) for untreated (control; upper panel) and RV16 treated (lower panel) and ( F ) showing the differentiated SAECs cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green), for the untreated (upper panel), RV16 treated (middle panel), and RV16 plus lipopolysaccharide (LPS) treated SAECs (lower panel). ( G ) Volcano plot of the up-regulated (red circles) and down-regulated (blue circles) genes comparing RV16 treated versus untreated SAECs as measured by Q-PCR. Labeled genes show at least a twofold change in expression at a significance level (α) of less than 0.05. ( H ) Heatmap of the significant differentially expressed genes for the RV16 treated SAECs ( n = 5) and untreated controls ( n = 3) showing low expression (green) and high expression (red) for the individual samples. Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Expressing, Control, Immunofluorescence, Microscopy, Labeling

    Exposure of the small airway epithelial cells (SAECs) in the model to cytomix (10 ng/ml each of tumor necrosis factor alpha [TNF-α], interferon gamma [IFN-γ], and interleukin-1 beta [IL-1β]). Confocal immunofluorescence microscopy images visualized using LASX 3D Viewer, of tight junctions (zonula occludens-1-AF647, red; E-cadherin-AF488, green) for untreated (control; left panel) and cytomix treated (right panel) and ( B ) showing the differentiated SAECs cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green) for the untreated (upper panel), cytomix treated SAECs (lower panel). ( C ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (control) and cytomix treated SAECs. Fold change in cytokine expression of SAECs following cytomix treatment compared to untreated control for ( D ) C–X–C motif chemokine ligand 10 (CXCL10), ( E ) C–C motif ligand 2 (CCL2), ( F ) C–C motif ligand 3 (CCL3), ( G ) interleukin-6 (IL-6), ( H ) IL-17, and ( I ) soluble receptor for advanced glycation end products (sRAGE) for 2 independent experiments with 4 replicates per experiment. Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Exposure of the small airway epithelial cells (SAECs) in the model to cytomix (10 ng/ml each of tumor necrosis factor alpha [TNF-α], interferon gamma [IFN-γ], and interleukin-1 beta [IL-1β]). Confocal immunofluorescence microscopy images visualized using LASX 3D Viewer, of tight junctions (zonula occludens-1-AF647, red; E-cadherin-AF488, green) for untreated (control; left panel) and cytomix treated (right panel) and ( B ) showing the differentiated SAECs cell types including cilia (acetylated tubulin-AF647, magenta), goblet cells (MUC5AC-AF405, blue), club cells (SCGB3A2-AF555, red), and basal cells (cytokeratin 5-AF488, green) for the untreated (upper panel), cytomix treated SAECs (lower panel). ( C ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (control) and cytomix treated SAECs. Fold change in cytokine expression of SAECs following cytomix treatment compared to untreated control for ( D ) C–X–C motif chemokine ligand 10 (CXCL10), ( E ) C–C motif ligand 2 (CCL2), ( F ) C–C motif ligand 3 (CCL3), ( G ) interleukin-6 (IL-6), ( H ) IL-17, and ( I ) soluble receptor for advanced glycation end products (sRAGE) for 2 independent experiments with 4 replicates per experiment. Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Immunofluorescence, Microscopy, Control, Expressing

    Exposure of the small airway epithelial cells (SAECs) in the model to pooled endotracheal aspirates (TA) from children with mild ( n = 10) and severe ( n = 10) pediatric acute respiratory distress syndrome (PARDS) samples. ( A ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (sham), mild PARDS TA, and severe PARDS TA treated SAECs. Fold change in cytokine expression from the epithelial (apical side) and endothelial (basal side) following mild and severe PARDS TA treatment compared to untreated (sham) SAECs for ( B ) C–X–C motif chemokine ligand 10 (CXCL10), ( C ) C–C motif ligand 2 (CCL2), ( D ) C–C motif ligand 3 (CCL3), ( E ) interleukin-6 (IL-6), ( F ) IL-17, and ( G ) soluble receptor for advanced glycation end products (sRAGE) One-way ANOVA with post-hoc Tukey test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Experiments were performed twice with three–four biological replicates per experiment.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Exposure of the small airway epithelial cells (SAECs) in the model to pooled endotracheal aspirates (TA) from children with mild ( n = 10) and severe ( n = 10) pediatric acute respiratory distress syndrome (PARDS) samples. ( A ) Transepithelial electrical resistance (Ω*cm 2 ) in untreated (sham), mild PARDS TA, and severe PARDS TA treated SAECs. Fold change in cytokine expression from the epithelial (apical side) and endothelial (basal side) following mild and severe PARDS TA treatment compared to untreated (sham) SAECs for ( B ) C–X–C motif chemokine ligand 10 (CXCL10), ( C ) C–C motif ligand 2 (CCL2), ( D ) C–C motif ligand 3 (CCL3), ( E ) interleukin-6 (IL-6), ( F ) IL-17, and ( G ) soluble receptor for advanced glycation end products (sRAGE) One-way ANOVA with post-hoc Tukey test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Experiments were performed twice with three–four biological replicates per experiment.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Expressing

    Drug testing using the small airway epithelial cell (SAEC)/human umbilical vein endothelial cell (HUVEC) model with cytomix as the inflammatory exposure. Measurement of ( A ) SAEC (epithelial) and ( B ) HUVECs (endothelial) expression of interleukin-6 (IL-6; pg/ml) in response to untreated (sham), cytomix (10 ng/ml each of tumor necrosis factor alpha [TNF-α], interferon gamma [IFN-γ], and interleukin-1 beta [IL-1β]), and cytomix plus the JAK1/2 inhibitor baricitinib (1 µM) for 24 h at 37 °C, 5% CO 2 . Independent experiments (n = 2), replicates per experiment (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Confocal immunofluorescence microscopy images ( C ) showing basal cells (cytokeratin 5-AF488, green; left panels), club cells (SCGB3A2-AF555, red; right panels) and pSTAT3 (green) and STAT3 (red) expression in SAECs for the untreated (top panels), cytomix (middle panels), and cytomix plus baricitinib treated (lower panels) SAECs. Images were taken at the same laser intensity for untreated, cytomix and cytomix + baricitinib treated samples.

    Journal: Scientific Reports

    Article Title: Development of an acute lung injury model for drug testing

    doi: 10.1038/s41598-025-02078-9

    Figure Lengend Snippet: Drug testing using the small airway epithelial cell (SAEC)/human umbilical vein endothelial cell (HUVEC) model with cytomix as the inflammatory exposure. Measurement of ( A ) SAEC (epithelial) and ( B ) HUVECs (endothelial) expression of interleukin-6 (IL-6; pg/ml) in response to untreated (sham), cytomix (10 ng/ml each of tumor necrosis factor alpha [TNF-α], interferon gamma [IFN-γ], and interleukin-1 beta [IL-1β]), and cytomix plus the JAK1/2 inhibitor baricitinib (1 µM) for 24 h at 37 °C, 5% CO 2 . Independent experiments (n = 2), replicates per experiment (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Confocal immunofluorescence microscopy images ( C ) showing basal cells (cytokeratin 5-AF488, green; left panels), club cells (SCGB3A2-AF555, red; right panels) and pSTAT3 (green) and STAT3 (red) expression in SAECs for the untreated (top panels), cytomix (middle panels), and cytomix plus baricitinib treated (lower panels) SAECs. Images were taken at the same laser intensity for untreated, cytomix and cytomix + baricitinib treated samples.

    Article Snippet: Primary human small airway epithelial cells ( SAECs ) isolated from normal human lung tissue in the distal portion of the lung (bronchiole area 1 mm) were purchased from Lonza (Basel, Switzerland) and expanded in PneumaCult-Ex Plus Media (StemCell Technologies, Vancouver, BC, Canada) in 75 cm 2 tissue culture flasks until 85% confluency.

    Techniques: Expressing, Immunofluorescence, Microscopy